Active Recombinant Rat Cd274 protein, His-tagged

• Cat.No.: Cd274-562R
• Product Overview: Recombinant Rat Cd274 protein(NP_001178883.1)(Met1-Thr238), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr238
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 His at 2 μg/ml (100 μl/well) can bind Rat PD-1 hFc, the EC50 of Rat PD-1 hFc is 15-50ng/mL.
• Molecular Mass: The recombinant rat CD274 comprises 232 amino acids and predicts a molecular mass of 26.5 kDa. The apparent molecular mass of the recombinant protein is approximately 50.7 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd274 CD274 molecule [ Rattus norvegicus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 molecule; programmed cell death 1 ligand 1; Pdcd1lg1; RGD1566211
• Gene ID: 499342
• mRNA Refseq: NM_001191954
• Protein Refseq: NP_001178883