Active Recombinant Rat CD28 protein, His-tagged

• Cat.No.: CD28-1876R
• Product Overview: Recombinant Rat CD28 protein(Asn 20 - Leu 150), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.
• Bio-activity: Loaded Biotinylated Human B7-1, Fc,Avitag on SA Biosensor, can bind Rat CD28, His Tag with an affinity constant of 15 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Biotinylated Human B7-2, Fc,Avitag on SA Biosensor, can bind Rat CD28, His Tag with an affinity constant of 6.1 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 16.9 kDa. The protein migrates as 30-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Asn20-Leu150
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: Asn20-Leu150

Gene Information

• Gene Name: Cd28 Cd28 molecule [ Rattus norvegicus ]
• Official Symbol: CD28
• Synonyms: CD28; Cd28 molecule; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD28RNA
• Gene ID: 25660
• mRNA Refseq: NM_013121
• Protein Refseq: NP_037253

Reviews

08/24/2021

Its unique characteristics contribute to obtaining clear and detailed structural information, enabling researchers to unravel the intricate complexities of protein interactions and conformational changes.

08/24/2021

Researchers can confidently rely on its capabilities to generate precise and significant results, enhancing the understanding of protein function and dynamics.

08/06/2021

the CD28 protein's outstanding performance in ELISA and its utility in protein electron microscopy structure analysis make it a highly recommended tool for researchers.

Q&As

Can CD28 protein be used for personalized medicine?

CD28 protein expression and function may be relevant for personalized treatment strategies in autoimmune diseases and cancer.

What are the potential clinical applications of targeting CD28 protein?

Targeting CD28 protein may have therapeutic potential in treating autoimmune diseases, cancer immunotherapy, and organ transplantation.

How does CD28 protein impact regulatory T cells (Tregs)?

CD28 signaling is important for the function and maintenance of regulatory T cells, which play a role in immune tolerance and suppression of autoimmunity.

How does CD28 protein impact cancer immunotherapy?

CD28 protein is involved in enhancing the anti-tumor immune response, making it a potential target for cancer immunotherapy.

Are there any approved therapies targeting CD28 protein?

Currently, there are no FDA-approved therapies specifically targeting CD28 protein, but research is ongoing in this area.