Active Recombinant Rat Chemokine (C-C motif) Ligand 20

• Cat.No.: Ccl20-409R
• Product Overview: Recombinantrat Ccl20 protein was expressed in E. coli. Ala26-Met96.

Specification

• Cat. No.: Ccl20-409R
• Description: Ccl20, also known asMIP3α, LARC (Liver and Activationregulated Chemokine) and as Exodus, is oneof many novel β chemokines identified through bioinformatics. Rat Ccl20 cDNAencodes a 96 amino acid (aa) precursor protein with a 25 aa putative signalpeptide that is predicted to be cleaved to form the 71 aa mature secretedprotein.
• Source: E. coli
• Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminal Sequence: Ala26
• Molecular Weight: 8.2 kDa
• Activity: Measured by itsability to chemoattract human CCR6 transfected BaF3 mouse pro-B cells. The ED50for this effect is typically 0.5-3 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 200μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month,2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions afterreconstitution.

Gene Information

• Gene Name: Ccl20 chemokine (C-C motif)ligand 20 [ Rattus norvegicus ]
• Official Symbol: Ccl20
• Synonyms: Ccl20; chemokine(C-C motif) ligand 20; ST38; Scya20; MIP-3-alpha; CCchemokine LARC; CC chemokine ST38; beta chemokine exodus-1; small-induciblecytokine A20; macrophage inflammatory protein 3 alpha; liver andactivation-regulated chemokine; beta-chemokine exodus-1; small induciblecytokine subfamily A20; C-C motif chemokine 20
• Gene ID: 29538
• mRNA Refseq: NM_019233
• Protein Refseq: NP_062106
• UniProt ID: P97884
• Chromosome Location: 9q34
• Pathway: Chemokine receptors bind chemokines; Chemokinesignaling pathway; Class A/1 (Rhodopsin-like receptors); Cytokine-cytokinereceptor interaction; G alpha (i) signalling events; GPCR downstreamsignaling; GPCR ligand binding; Peptide ligand-binding receptors; Rheumatoidarthritis; Signal Transduction; Signaling by GPCR
• Function: chemokine activity; cytokine activity