Active Recombinant Rat FAP protein, His-tagged

Species :	Rat
Source :	HEK293
Tag :	His
Protein Length :	26-761 a.a.
Form :	Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
Bio-activity :	Immobilized Rat FAP, His Tag at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.6-4 ng/mL.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >6500 pmol/min/μg.
Molecular Mass :	This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 91.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Proteinlength :	Leu26-Asp761
Endotoxin :	Less than 1.0 EU per μg by the LAL method.
Purity :	>95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
Storage :	For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	Fap fibroblast activation protein, alpha [ Rattus norvegicus ]
Official Symbol	FAP
Synonyms	FAP; fibroblast activation protein, alpha; seprase;
Gene ID	192203
mRNA Refseq	NM_138850
Protein Refseq	NP_620205