Active Recombinant Rat IGF1R protein, His-tagged

• Cat.No.: IGF1R-0287R
• Product Overview: Recombinant Rat IGF1R protein(Glu31-His936), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 31-936 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat IGF-I R, His Tag at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 5-39 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.
  This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and 24.2 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu31-His936
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Igf1r insulin-like growth factor 1 receptor [ Rattus norvegicus ]
• Official Symbol: IGF1R
• Synonyms: IGF1R; insulin-like growth factor 1 receptor; IGF-I receptor; insulin-like growth factor I receptor; JTK13; IGFIRC
• Gene ID: 25718
• mRNA Refseq: NM_052807
• Protein Refseq: NP_434694