Active Recombinant Rat Il12b&Il23a

• Cat.No.: Il12b&Il23a-747R
• Product Overview: Recombinant Rat Il12b&Il23a (Accession # NP_072133 (p40) & NP_569094 (p19)), fused with , was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Species: Rat
• Source: Sf21 Cells
• Tag: Non
• Predicted N Terminal: Met23
• Form: Lyophilized from a 0.2 µm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.The ED50 for this effect is typically 0.1 - 0.5 ng/mL.
• Molecular Mass: Recombinant Rat Il12b&Il23a has a calculated MW of 58.3 kDa. In SDS-PAGE migrates as 66 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 50 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Il12b interleukin 12B [ Rattus norvegicus ]
• Official Symbol: Il12b
• Synonyms: IL12B; interleukin 12B; interleukin-12 subunit beta; interleukin-12 p40; Il12
• Gene ID: 64546
• mRNA Refseq: NM_022611
• Protein Refseq: NP_072133