Active Recombinant Rat Il1rn protein, hFc-tagged

• Cat.No.: Il1rn-8709R
• Product Overview: Recombinant Rat Il1rn protein(P25086)(His27-Gln178), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: 27-178 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Immobilized rat IL1R1-His at 10 μg/ml (100 μl/well) can bind rat Fc-IL1RN, The EC50 of rat Fc-IL1RN is 5.1-11.9 ng/ml.
• Molecular Mass: The recombinant rat IL1RN comprises 412 amino acids and predicts a molecular mass of 45.8 kDa. The apparent molecular mass of the recombinant protein is approximately 47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Proteinlength: His27-Gln178
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il1rn interleukin 1 receptor antagonist [ Rattus norvegicus ]
• Official Symbol: Il1rn
• Synonyms: IL1RN; interleukin 1 receptor antagonist; interleukin-1 receptor antagonist protein; IRAP; IL-1RN; IL1 inhibitor; IL-1ra
• Gene ID: 60582
• mRNA Refseq: NM_022194
• Protein Refseq: NP_071530