Active Recombinant Rat IL2RB protein, His&Twin-Strep-tagged

• Cat.No.: IL2RB-9511R
• Product Overview: Recombinant Rat IL2RB protein(Ala 27 - Glu 239 (IL-2RB) & Ser 25 - Ala 262 (IL-2RG)), fused with His and Twin-Strep tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His&Twin-Strep
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Loaded Biotinylated Human IL-2, Fc,Avitag on SA Biosensor, can bind Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag with an affinity constant of 3.50 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: Molecular CharacterizationRat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 30.2 kDa (IL-2RB) and 34.7 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 40-50 kDa and 55-65 kDa respectively due to glycosylation.
• Protein Length: Ala 27 - Glu 239 (IL-2RB) & Ser 25 - Ala 262 (IL-2RG)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il2rb interleukin 2 receptor, beta [ Rattus norvegicus ]
• Official Symbol: IL2RB
• Synonyms: IL2RB; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; IL-2RB; P70-75; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 2 receptor, beta chain; high affinity IL-2 receptor subunit beta; IL2RBC
• Gene ID: 25746
• mRNA Refseq: NM_013195
• Protein Refseq: NP_037327