Active Recombinant Rat Interleukin 2 Receptor, Alpha

• Cat.No.: Il2ra-474R
• Product Overview: Recombinant Ratll2ra protein was expressed in Chinese Hamster Ovary cell line, CHO-derived. Glu22- Gln235.

Specification

• Cat. No.: Il2ra-474R
• Description: Il2ra, also known asCD25, is a 55 kDa type I membrane glycoprotein that belongs to the family ofcytokine receptors that utilize the common gamma chain subunit (γc). Il2ra isprimarily expressed on activated T cells and on regulatory T cells (Treg). Therat Il2ra cDNA encodes a 267 amino acid (aa) precursor that includes a 21 aasignal peptide, a 214 aa extracellular domain (ECD) with two Sushi domains, a21 aa transmembrane segment, and an 11 aa cytoplasmic domain. Within the ECD,rat Il2ra shares 58% and 81% aa sequence identity with human and mouse IL2 Rα,respectively. It shares approximately 15% aa sequence identity with IL4, 7, 9,15, and 21 receptor subunits that also complex with γc.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Glu22
• MolecularWeight: 51.6 kDa (monomer)
• Activity: Measured by itsability to inhibit the IL2dependent proliferation of MO7e human megakaryocyticleukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50for this effect is typically 0.35 1.4 μg/mL in the presence of 30 ng/mL of rhIL2.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >95%, by SDSPAGE underreducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 500μg/mL in sterile PBS.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il2ra interleukin 2 receptor,alpha [ Rattus norvegicus ]
• Official Symbol: Il2ra
• Synonyms: Il2ra; interleukin 2receptor, alpha; IL2RAC; interleukin-2 receptor subunit alpha; IL2-RA; IL-2-RA;IL-2R subunit alpha; IL-2 receptor alpha subunit; IL-2 receptor subunit alpha;interleukin-2 receptor alpha chain; interleukin 2 receptor, alpha chain
• Gene ID: 25704
• mRNA Refseq: NM_013163
• Protein Refseq: NP_037295
• Chromosome Location: 17q12.3
• Pathway: Cytokine Signalingin Immune system; Cytokine-cytokine receptor interaction; Endocytosis; HTLV-Iinfection; Hematopoietic cell lineage; IL-2 Signaling Pathway; Immune System;Inflammatory Response Pathway; Interleukin receptor SHC signaling;Interleukin-2 signaling; Interleukin-3, 5 and GM-CSF signaling; Jak-STATsignaling pathway; Measles; Signaling by Interleukins
• Function: drug binding;interleukin-2 binding; interleukin-2 receptor activity; receptor activity