Active Recombinant Rat Pcsk9 protein, His-tagged

• Cat.No.: Pcsk9-915R
• Product Overview: Recombinant Rat Pcsk9 protein(Gln 31 - Gln 691), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Gln 31 - Gln 691
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Bio-activity: Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Rat PCSK9, His Tag with a linear range of 0.156-0.625 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 59.3 kDa respectively. The protein migrates as 16 kDa and 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pcsk9 proprotein convertase subtilisin/kexin type 9 [ Rattus norvegicus ]
• Official Symbol: Pcsk9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; proprotein convertase 9; proprotein convertase PC9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; neural apoptosis-regulated convertase 1; PC9; Narc1; NARC-1
• Gene ID: 298296
• mRNA Refseq: NM_199253
• Protein Refseq: NP_954862