Active Recombinant Rat PVR protein, His-tagged

• Cat.No.: PVR-3325R
• Product Overview: Recombinant Rat PVR protein(NP_058772.2)(Met1-Gly352), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gly352
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized rat CD155 at 20 μg/ml (100 μl/well) can bind mouse CD226 with a linear range of 6.4-160 ng/ml.
• Molecular Mass: The recombinant rat PVR comprises 330 amino acids and predicts a molecular mass of 35.8 kDa. As a result of glycosylation, the apparent molecular mass of the rat PVR is approximately 55-65 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PVR poliovirus receptor [ Rattus norvegicus ]
• Official Symbol: PVR
• Synonyms: PVR; poliovirus receptor; tumor-associated antigen 1; tumor-associated glycoprotein pE4; Taa1; Tage4
• Gene ID: 25066
• mRNA Refseq: NM_017076
• Protein Refseq: NP_058772