Active Recombinant Rat SELE protein, hFc-tagged

• Cat.No.: SELE-1977R
• Product Overview: Recombinant Rat SELE protein(NP_620234.1)(Met1-Pro494), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 cells.When 5 x 10E4 cells/well are added to SELE-coated plates (2 μg/mL and 100 μL/well), approximately >85% cells will adhere specifically after 60 minutes at 37℃.
• Molecular Mass: The recombinant rat SELE/Fc is a disulfide-linked homodimer. The reduced monomer comprises 714 amino acids and has a predicted molecular mass of 78.8 kDa. The apparent molecular mass of the protein is approximately 114-119 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Pro494
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sele selectin E [ Rattus norvegicus ]
• Official Symbol: SELE
• Synonyms: SELE; selectin E; E-selectin; ELAM-1; LECAM2; selectin, endothelial cell; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2
• Gene ID: 25544
• mRNA Refseq: NM_138879
• Protein Refseq: NP_620234