| Species : |
Rat |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
24-257 aa |
| Description : |
Siglec-15 is a transmembrane glycoprotein belonging to the Siglec family, recognized for its sialic acid-binding properties and role in immune regulation. Structurally, its extracellular domain contains two Ig-like domains, and it functionally associates with the signaling adaptors DAP12 and DAP10. Siglec-15 is expressed on immune cells, including osteoclasts, macrophages, and dendritic cells. It exerts its function by binding to its ligand, the sialyl-Tn antigen. This interaction is critical for inducing TGF-beta production by tumor-associated macrophages and plays an essential role in osteoclast formation and TRANCE/RANK Ligand signaling. |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant. |
| Bio-activity : |
Immobilized Rat Siglec-15 Protein, His Tag at 1 μg/mL (100 μL/well) can bind Mouse Human chimeric mAb (5G12, Human IgG1) with a linear range of 0.06-2 ng/mL. |
| Molecular Mass : |
The protein has a calculated MW of 27.4 kDa. The protein migrates as 32-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU/μg by the LAL method/rFC method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
