Active Recombinant Rat Tfrc Protein, His-tagged

• Cat.No.: Tfrc-11163R
• Product Overview: Rat Transferrin R, His Tag is expressed from human 293 cells (HEK293). It contains AA Gly 87 - Phe 761 (Accession # Q99376-1). This protein carries a polyhistidine tag at the N-terminus. Predicted N-terminus: His

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 87-761 aa
• Description: CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71/TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC/TfR has been shown to interact with GABARAP and HFE.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Rat Transferrin R, His Tag at 5 μg/mL (100 μL/well) can bind Mouse Transferrin, Mouse IgG2a Fc Tag with a linear range of 0.3-20 ng/mL.
• Molecular Mass: The protein has a calculated MW of 78.1 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Tfrc transferrin receptor [ Rattus norvegicus ]
• Official Symbol: Tfrc
• Synonyms: TFRC; transferrin receptor; transferrin receptor protein 1; TR; tfR; tfR1; Trfr
• Gene ID: 64678
• mRNA Refseq: NM_022712
• Protein Refseq: NP_073203
• UniProt ID: Q99376