| Description : |
Tumor Necrosis Factor-Alpha (TNF-Alpha) also known as Cachectin and TNFSF1A, plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease. |
| Source : |
P. pastoris |
| Species : |
Rat |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 50 pg/mL, measured in a cytotoxicity assay using mouse L-929 cells in the presence of actinomycin D, corresponding to a specific activity of > 2.0 × 10^7 units/mg. |
| Molecular Mass : |
17.4kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
157 |
| AA Sequence : |
MLRSSSQNSSDKPVAHVVANHQAEE QLEWLSQRANALLANGMDLKDNQLV VPADGLYLIYSQVLFKGQGCPDYVL LTHTVSRFAISYQEKVSLLSAIKSP CPKDTPEGAELKPWYEPMYLGGVFQ LEKGDLLSAEVNLPKYLDITESGQV YFGVIAL |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% by SDS-PAGE and HPLC analyses. |
| Storage : |
Lyophilized recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rrTNF-Alpha should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
