Active Recombinant Rat Vegfa protein, His-tagged, 27-190aa

• Cat.No.: Vegfa-38R
• Product Overview: Recombinant Rat Vegfa protein(27-190aa), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 1mM DTT, 5% trehalose and Proclin300.
• Bio-activity: Vascular endothelial growth factor A (VEGF-A), a glycosylated mitogen, is known to be a vascular permeability factor and an endothelial cell growth factor secreted by the smooth muscle and endothelial cells. It has been reported that VEGF-A induces vascular permeability and growth, promotes monocyte/macrophage migration, and inhibits cell apoptosis and so on. To test the effect of VEGF-A on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of VEGFA. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell
  Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .
• Molecular Mass: 22kDa
• Protein length: 27-190aa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >98%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Vegfa vascular endothelial growth factor A [ Rattus norvegicus ]
• Official Symbol: Vegfa
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vegf; VEGF164
• Gene ID: 83785
• mRNA Refseq: NM_001110333
• Protein Refseq: NP_001103803

Reviews

04/21/2021

VEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.

03/09/2020

VEGFA they produced was highly active and the experimental effect was obvious.

Q&As

How does VEGFA protein relate to other biomarkers?

Studies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.

How to harness the activity of VEGFA protein for disease treatment?

At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.

What is the role of VEGFA protein in tumor angiogenesis?

VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.

How well is the VEGFA protein expressed in different tissues and organs?

The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.

What is the relationship between the expression level of VEGFA protein and the degree of tumor malignancy?

Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.

How about the fuction of VEGFA protein in cardiovascular disease?

VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.