Active Recombinant Rhesus macaque DKK1 protein(256Asn/Gln), His-tagged

• Cat.No.: DKK1-2339R
• Product Overview: Active Recombinant Rhesus macaque DKK1 protein(XP_001098844.1)(Met 1-His 266) (256 Asn / Gln) was expressed in HEK293, fused with a C-terminal polyhistidine tag.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: 1-266 aa, 256Asn/Gln
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 0.5-3 μg/ml in the presence of 10 ng/mL of Wnt3a.
• Molecular Mass: The recombinant rhesus DKK1 comprises 246 amino acids with a predicted molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhesus DKK1 is approximately 38-43 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.