Active Recombinant Rhesus IL1B Protein
Cat.No. : | IL1B-1167R |
Product Overview : | Recombinant mature form of Rhesus IL1B (Ala 117-Ser 269) was expressed and purified, with an initial Met. |
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Species : | Rhesus macaque |
Source : | E.coli |
Tag : | Non |
Protein Length : | Ala117-Ser269 |
Form : | Lyophilized from sterile 50 mM Tris, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.25-1 ng/mL. |
Molecular Mass : | The recombinant rhesus IL1B consists of 154 amino acids and has a calculated molecular mass of 17.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions. |
Purity : | > 97 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
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Not For Human Consumption!
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