Active Recombinant Rhesus IL1B Protein

• Cat.No.: IL1B-1167R
• Product Overview: Recombinant mature form of Rhesus IL1B (Ala 117-Ser 269) was expressed and purified, with an initial Met.

Specification

• Source: E. coli
• Species: Rhesus
• Tag: N/A
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.25-1 ng/mL.
• Molecular Mass: The recombinant rhesus IL1B consists of 154 amino acids and has a calculated molecular mass of 17.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Ala117-Ser269
• Purity: > 97 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.