Active Recombinant Rhesus macaque CDH17 protein, His-tagged, Low Endotoxin

• Cat.No.: CDH17-1337R
• Product Overview: Recombinant Rhesus macaque CDH17 protein(A0A1D5R2B4)(23-784 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: 23-784 aa
• Form: Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque CDH17, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CDH17 Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 85.60 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.