Active Recombinant Rhesus macaque EGFR protein, His-tagged

• Cat.No.: EGFR-61C
• Product Overview: Recombinant Rhesus macaque EGFR Protein (25-645aa), was expressed in HEK293 with C-terminal His tag.

Specification

• Species: Rhesus macaque
• Source: HEK293
• Tag: His
• Protein Length: 25-645 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rhesus macaque EGF R, His Tag at 2 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.2-3 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 70.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu25-Ser645
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.