Active Recombinant Rhesus macaque VTCN1 Protein, His-tagged

• Cat.No.: VTCN1-1519R
• Product Overview: Recombinant Rhesus macaque B7-H4/VTCN1 Protein (Phe29-Ala258) with a C-His tag was expressed in human cells.

Specification

• Description: B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins.It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.
• Source: Human Cells
• Species: Rhesus macaque
• Tag: His
• Form: Lyophilized
• Bio-activity: Immobilized Human GFRA1-His at 0.5µg/ml(100 µL/well) can bind Human GDNF. The ED50 of GDNF is 14.73 µg/mL.
• Molecular Mass: 26.4 kDa
• Endotoxin: < 1.0 EU per µg as determined by the LAL method.
• Purity: > 95% as determined by reducing SDS-PAGE.
• Applications: Immunogen
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Storage Buffer: Lyophilized from a 0.2 µm filtered solution of PBS, pH7.4.
• Reconstitution: Please refer to the printed manual for detailed information.
• Shipping: Ice packs.
• Protein length: Phe29-Ala258
• Publication:
  Discovery and Preclinical Characterization of XMT-1660, an Optimized B7-H4-Targeted Antibody-Drug Conjugate for the Treatment of Cancer (2023)

Gene Information

• Gene Name: VTCN1 V-set domain containing T cell activation inhibitor 1 [ Macaca mulatta (Rhesus monkey) ]
• Official Symbol: VTCN1
• Synonyms: VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1
• Gene ID: 714123
• mRNA Refseq: XM_001103715
• Protein Refseq: XP_001103715
• UniProt ID: F7B770