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Recombinant Mouse Vtcn1 Protein, Fc-tagged

Cat.No.: Vtcn1-825M
Product Overview: Recombinant mature form of mouse B7H4 (Q7TSP5) (phe 29-Ser 256) was fused with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Mouse
Tag: Fc
Predicted N Terminal: phe 29
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The secreted recombinant mouse B7H4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 469 amino acids and has a calculated molecular mass of 52.2 kDa. The apparent molecular mass of rmB7H4/Fc monomer is approximately 70-75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Vtcn1 V-set domain containing T cell activation inhibitor 1 [ Mus musculus ]
Official Symbol: Vtcn1
Synonyms: VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; t cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7x; B7h4; B7s1; BC032925; MGC41287;
Gene ID: 242122
mRNA Refseq: NM_178594
Protein Refseq: NP_848709
MIM:
UniProt ID:
Function: receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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