Active Recombinant Saccharomyces cerevisiae KEX2 Protein

• Cat.No.: KEX2-01S
• Product Overview: Recombinant Kex2 Protease is a genetically engineered protein expressed in Pichia pastoris. The amino acid sequence of recombinant Kex2 Protease is identical to Saccharomyces cerevisiae Kex2

Specification

• Description: Processing of precursors of alpha-factors and killer toxin.
• Source: Yeast
• Species: Saccharomyces cerevisiae
• Form: Lyophilized powder
• Bio-activity: ≥ 10.0 units/mg protein
• Molecular Mass: 67.0±6.7 kDa
• Unit Definition: One unit of Kex2 activity will release 1 μmol 4-nitroaniline per minute in a reaction volume of 3.0 mL at pH 8.0 and 25 centigrade with Boc-QRR-pNA (Boc-Gln-Arg-Arg-pNA) as the substrate.
• Usage: Recommended reaction buffer: pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+ or HEPES, 5 mM Ca2+. If it is not used immediately after dissolving, it is recommended to dissolve the powder with 20 mM (pH 5.2) NaAc-HAc and 2 mM Ca2+ buffer to make the final concentration is about 1-10 mg/ml, then make suitable packing as your requirement and store at -20 centigrade. Reaction was carried out, using pH 7.0-9.0, 50 mM Tris-HCl, 2 mM Ca2+ or HEPES, 5 mM Ca2+ buffer.
  Note: The optimum reaction pH of the enzyme is pH 9.0 and the optimum stable pH is 5.0-6.0.
• Stability: Stability of storage: Recombinant kex2 protease should be stored under 2-8 centigrade in sealed container. It is stable within 12 months. After dissolved with 20mM NaAc-HAc, pH 5.2, 2mM Ca2+, it should be stored under -20 centigrade.
  Stability of transport: The product is stable by blue ice insulation transport.
• Storage: 2-8 centigrade, sealed, dark

Gene Information

• Gene Name: KEX2 kexin KEX2 [ Saccharomyces cerevisiae S288C ]
• Official Symbol: KEX2
• Synonyms: KEX2; kexin KEX2; QDS1; SRB1; VMA45; kexin KEX2; EC 3.4.21.61; Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
• Gene ID: 855483
• mRNA Refseq: NM_001183076
• Protein Refseq: NP_014161
• UniProt ID: P13134