Active Recombinant Serratia marcescens Universal Nuclease

• Cat.No.: Nuclease-37S
• Product Overview: Universal Nuclease is an endonuclease derived from Serratia marcescens and is expressed by yeast system, and is suitable for scenarios where nucleic acids need to be removed from various proteins and other biological products. The reaction conditions are mild, and it has a very high nucleic acid removal efficiency. Function Testing: Complete degradation of DNA Protease Activity: Not detected

Specification

• Species: Serratia marcescens
• Source: Yeast
• Tag: Non
• Description: Universal Nuclease can completely digest all forms of DNA and RNA (including single-stranded, double-stranded, linear, and circular) into 5'-monophosphorylated oligonucleotides with a length of 3-5 bases. It has no protease activity and high specificity.
• Form: Transparent liquid. Colorless
• Purity: ≥ 95% by SDS-PAGE
• Bio-activity: ≥ 250 U/μL
• Advantages: 1. It features mild reaction conditions and high stability. 2. There are two types available, His Tag and Tag free, which are suitable for a variety of purification methods.
• Unit definition: One unit (U) of the enzyme is defined as the amount that can digest sonicated salmon sperm DNA into acid-soluble oligonucleotides equivalent to 1.0 ΔA260 within 30 minutes at pH 8.0 and 37 centigrade in a reaction volume of 2.625 mL.
• Endotoxin: < 0.2 EU/1000U
• Usage: 1. Universal Nuclease can be added during cell lysis to decrease the viscosity of the lysate. The recommended dosage is 25 U/mL. 2. It can eliminate residual nucleic acids from protein samples during purification or from finished protein products. The recommended dosage is also 25 U/mL. 3. The activity of this product requires 1-2 mM Mg2+. Meanwhile, avoid having the NaCl concentration exceed 100mM.
• Applications: 1. Cell lysis 2. Protein chromatography 3. Nucleic acid removal from biological products
• Note: 1. Try to avoid freeze-thaw cycles of this product after receipt; 2. Please wear lab coat and disposable gloves when using; 3. This product should not be used directly for clinical diagnosis and treatment.
• Stability: This product should be stored at -20 centigrade and can be stored for at least 24 months.
• Storage: Store at -20 centigrade.
• Storage Buffer: 20 mM Tris-HCl, 2 mM MgCl2, 20 mM NaCl, 50% glycerol, pH = 8.0
• Shipping: Dry Ice