| Description : |
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also produced from muscle, and is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10. |
| Source : |
Yeast |
| Species : |
Swine |
| Form : |
Lyophilized without carrier protein. |
| Bio-activity : |
The biological activity of recombinant swine IL-6 was measured in a cell proliferation assay using the mouse B9 cell line. The ED50 for this effect is typically 1-10 pg/mL. |
| Molecular Mass : |
Swine IL-6 has a predicted molecular weight of 20.9 kDa. |
| AA Sequence : |
GRLEEDAKGD ATSDKMLFTS PDKTEELIKY ILGKISAMRK EMCEKYEKCE NSKEVLAENN LNLPKMAEKD GCFQSGFNQE TCLMRITTGL VEFQIYLDYL QKEYESNKGN VEAVQISTKA LIQTLRQKGK NPDKATTPNP TTNAGLLDKL QSQNEWMKNT KIILILRSLE DFLQFSLRAI RIM (183) |
| Purity : |
>95% as visualized by SDS-PAGE analysis |
| Applications : |
The Swine IL-6 protein can be used in cell culture, as an IL-6 ELISA Standard, and as a Western Blot Control. |
| Storage : |
Stable for up to twelve months from date of receipt at -20 oC. Stable for at least 3 months when stored in working aliquots with a carrier protein at -20 oC. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein. |
