Active Recombinant Zebrafish BMP4 Protein

• Cat.No.: BMP4-14Z
• Product Overview: Recombinant Zebrafish BMP4 Protein was expressed in E. coli.

Specification

• Description: Predicted to enable BMP receptor binding activity and cytokine activity. Acts upstream of or within several processes, including animal organ development; determination of bilateral symmetry; and embryonic morphogenesis. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including cardiovascular system; fin; head; inner ear; and mesoderm. Human ortholog(s) of this gene implicated in several diseases, including CAKUT (multiple); cleft lip; orofacial cleft 11; ossification of the posterior longitudinal ligament of spine; and otosclerosis. Orthologous to human BMP4 (bone morphogenetic protein 4).
• Source: E. coli
• Species: Zebrafish
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.2-1 µg/mL.
• Molecular Mass: 12.9 kDa
• Protein Length: Ser288-Arg400
• N-terminal Sequence Analysis: Met
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Related Research Areas: BMP Family
  BMPs
  BMPs (Bone Morphogenetic Proteins)
  BMPs and Regulators Secreted by Endothelial Cells
  Cardiac Stem Cell Markers
  Early Ectodermal Lineage Markers
  Early Mesodermal Lineage Markers
  EMT Induction
  Growth Factors
  Molecules Secreted by VSMC
  Neural Crest Cell Markers
  Search Cytokine and Growth Factor Products
  TGF-beta Superfamily

Gene Information

• Gene Name: bmp4 bone morphogenetic protein 4 [ Danio rerio (zebrafish) ]
• Official Symbol: BMP4
• Synonyms: BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME
• Gene ID: 30612
• mRNA Refseq: NM_131342.2
• Protein Refseq: NP_571417.1
• UniProt ID: O57574