Active Recombinant Zebrafish VEGF165 Protein, Alexa Fluor 555 conjugated

• Cat.No.: VEGF165-1026ZAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified.

Specification

• Species: Zebrafish
• Source: Insect Cells
• Protein Length: 165
• Form: Lyophilized
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 10-30 ng/mL.
• Molecular Mass: The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 kDa band in SDS-PAGE under reducing conditions.
• N-terminal Sequence Analysis: Ala 24
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to-70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.01, 5%-8% trehalose and mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 555

Gene Information

• Gene Name: vegfaa vascular endothelial growth factor Aa [ Danio rerio (zebrafish) ]
• Official Symbol: vegfaa
• Synonyms: vegfaa; vascular endothelial growth factor Aa; vegf; vegfa; wu:fj82c06; vascular endothelial growth factor A-A; VEGF-A-A; etID309976.16; vascular endothelial growth factor A; vegf-a
• Gene ID: 30682
• mRNA Refseq: NM_131408
• Protein Refseq: NP_571483
• UniProt ID: O73682