Akt1/PKBα Immunoprecipitation-Kinase Assay Kit
Cat.No. : | Kit-0067 |
Product Overview : | Akt1/PKBα Immunoprecipitation-Kinase Assay Kit designed to measure phosphotransferase activity in an immunocomplex formed between the Akt1/PKBa PH Domain antibody & active Akt/PKB. |
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Size : | 1 kit |
Applications : | Application: Akt1/PKBα Immunoprecipitation-Kinase Assay Kit designed to measure phosphotransferase activity in an immunocomplex formed between the Akt1/PKBa PH Domain antibody & active Akt/PKB.Key Applications: Kinase Assay; Immunoprecipitation. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (9)
Ask a questionPKB protein is regulated through a complex network of signaling pathways. The activation of PKB is primarily mediated by phosphoinositide 3-kinase (PI3K) signaling pathway. Activation of PI3K leads to the production of phosphatidylinositol (3,4,5)-trisphosphate (PIP3), which recruits PKB to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent protein kinase-1 (PDK1) at Thr308. Full activation of PKB requires further phosphorylation at Ser473 by the mammalian target of rapamycin complex 2 (mTORC2) or other kinases.
There are three isoforms of PKB protein known as PKBα (AKT1), PKBβ (AKT2), and PKBγ (AKT3). These isoforms show high sequence homology and share similar functions but may have distinct roles in specific tissues or under certain conditions.
Yes, PKB plays a crucial role in neuronal function. It is involved in promoting neuronal survival, growth, and differentiation. PKB signaling pathway is also essential for synaptic plasticity, memory formation, and neurotransmitter receptor trafficking, which are necessary for proper neuronal communication and function.
PKB protein phosphorylates and regulates numerous downstream targets involved in different cellular processes. Some well-known targets include proteins involved in promoting cell survival (e.g., BAD, caspase-9), stimulating protein synthesis (e.g., mTOR), regulating glucose metabolism (e.g., GLUTs), and modulating cell cycle progression (e.g., p27Kip1).
PKB protein is activated in response to various extracellular signals, including growth factors (e.g., insulin-like growth factor-1, epidermal growth factor), hormones (e.g., insulin), and cytokines. Activation of PKB involves phosphorylation on specific residues, particularly Thr308 and Ser473, which leads to its conformational changes and subsequent activation.
Yes, dysregulation of PKB protein has been implicated in various diseases, including cancer, diabetes, cardiovascular diseases, and neurological disorders. Abnormal activation or inactivation of PKB can disrupt normal cellular processes and contribute to disease development.
Yes, PKB is a critical mediator of insulin signaling. Insulin stimulates the activation of PKB through the PI3K pathway, resulting in increased glucose uptake and metabolism, as well as inhibition of gluconeogenesis in the liver. PKB also promotes the translocation of glucose transporter proteins (GLUTs) to the cell membrane, facilitating glucose uptake.
Dysregulated PKB signaling has been implicated in various aspects of cancer development. Overactivation of PKB can promote cell proliferation, survival, angiogenesis, and metastasis, while inhibiting apoptosis. Mutations or genetic alterations affecting the PTEN (Phosphatase and Tensin Homolog) tumor suppressor, which negatively regulates PKB signaling, are commonly found in many cancers. The activation of PKB in cancer cells is often associated with poor prognosis and resistance to cancer therapies.
Several inhibitors targeting PKB protein, such as MK-2206 and GSK2141795, have been developed and tested in preclinical and clinical studies. These inhibitors aim to block the activity of PKB and potentially provide therapeutic benefits in diseases where aberrant PKB signaling is involved.
Customer Reviews (4)
Write a reviewThe PKB protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.
With its high purity and stability, this protein ensures dependable and accurate results, which are crucial for the success of my research endeavors.
In addition to its impeccable quality, the manufacturer of the PKB protein provides outstanding technical support that can efficiently resolve any challenges encountered during my experiments.
Whether it is for investigating cellular pathways, studying protein functions, or exploring signaling cascades, the PKB protein offers unparalleled versatility and performance.
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