Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Akt1/PKBα Immunoprecipitation-Kinase Assay Kit

Cat.No. : Kit-0067
Product Overview : Akt1/PKBα Immunoprecipitation-Kinase Assay Kit designed to measure phosphotransferase activity in an immunocomplex formed between the Akt1/PKBa PH Domain antibody & active Akt/PKB.
  • Specification
  • Gene Information
  • Related Products
Size : 1 kit
Applications : Application: Akt1/PKBα Immunoprecipitation-Kinase Assay Kit designed to measure phosphotransferase activity in an immunocomplex formed between the Akt1/PKBa PH Domain antibody & active Akt/PKB.Key Applications: Kinase Assay; Immunoprecipitation.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (9)

Ask a question
How is PKB protein regulated? 04/29/2023

PKB protein is regulated through a complex network of signaling pathways. The activation of PKB is primarily mediated by phosphoinositide 3-kinase (PI3K) signaling pathway. Activation of PI3K leads to the production of phosphatidylinositol (3,4,5)-trisphosphate (PIP3), which recruits PKB to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent protein kinase-1 (PDK1) at Thr308. Full activation of PKB requires further phosphorylation at Ser473 by the mammalian target of rapamycin complex 2 (mTORC2) or other kinases.

How many isoforms of PKB protein are there? 01/25/2023

There are three isoforms of PKB protein known as PKBα (AKT1), PKBβ (AKT2), and PKBγ (AKT3). These isoforms show high sequence homology and share similar functions but may have distinct roles in specific tissues or under certain conditions.

Is PKB protein involved in neuronal function? 06/26/2021

Yes, PKB plays a crucial role in neuronal function. It is involved in promoting neuronal survival, growth, and differentiation. PKB signaling pathway is also essential for synaptic plasticity, memory formation, and neurotransmitter receptor trafficking, which are necessary for proper neuronal communication and function.

What are the downstream targets of PKB protein? 04/18/2021

PKB protein phosphorylates and regulates numerous downstream targets involved in different cellular processes. Some well-known targets include proteins involved in promoting cell survival (e.g., BAD, caspase-9), stimulating protein synthesis (e.g., mTOR), regulating glucose metabolism (e.g., GLUTs), and modulating cell cycle progression (e.g., p27Kip1).

What activates PKB protein? 02/23/2021

PKB protein is activated in response to various extracellular signals, including growth factors (e.g., insulin-like growth factor-1, epidermal growth factor), hormones (e.g., insulin), and cytokines. Activation of PKB involves phosphorylation on specific residues, particularly Thr308 and Ser473, which leads to its conformational changes and subsequent activation.

Can dysregulation of PKB protein contribute to disease development? 10/20/2019

Yes, dysregulation of PKB protein has been implicated in various diseases, including cancer, diabetes, cardiovascular diseases, and neurological disorders. Abnormal activation or inactivation of PKB can disrupt normal cellular processes and contribute to disease development.

Does PKB play a role in insulin signaling? 02/17/2019

Yes, PKB is a critical mediator of insulin signaling. Insulin stimulates the activation of PKB through the PI3K pathway, resulting in increased glucose uptake and metabolism, as well as inhibition of gluconeogenesis in the liver. PKB also promotes the translocation of glucose transporter proteins (GLUTs) to the cell membrane, facilitating glucose uptake.

How is PKB involved in cancer? 10/20/2017

Dysregulated PKB signaling has been implicated in various aspects of cancer development. Overactivation of PKB can promote cell proliferation, survival, angiogenesis, and metastasis, while inhibiting apoptosis. Mutations or genetic alterations affecting the PTEN (Phosphatase and Tensin Homolog) tumor suppressor, which negatively regulates PKB signaling, are commonly found in many cancers. The activation of PKB in cancer cells is often associated with poor prognosis and resistance to cancer therapies.

Are there any drugs or inhibitors targeting PKB protein? 03/12/2017

Several inhibitors targeting PKB protein, such as MK-2206 and GSK2141795, have been developed and tested in preclinical and clinical studies. These inhibitors aim to block the activity of PKB and potentially provide therapeutic benefits in diseases where aberrant PKB signaling is involved.

Customer Reviews (4)

Write a review
Reviews
04/23/2023

    The PKB protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.

    03/09/2021

      With its high purity and stability, this protein ensures dependable and accurate results, which are crucial for the success of my research endeavors.

      09/13/2020

        In addition to its impeccable quality, the manufacturer of the PKB protein provides outstanding technical support that can efficiently resolve any challenges encountered during my experiments.

        06/14/2017

          Whether it is for investigating cellular pathways, studying protein functions, or exploring signaling cascades, the PKB protein offers unparalleled versatility and performance.

          Ask a Question for All AKT/PKB Products

          Required fields are marked with *

          My Review for All AKT/PKB Products

          Required fields are marked with *

          0

          Inquiry Basket

          cartIcon
          logo

          FOLLOW US

          Terms and Conditions        Privacy Policy

          Copyright © 2024 Creative BioMart. All Rights Reserved.

          Contact Us

          • /

          Stay Updated on the Latest Bioscience Trends