Alanine Aminotransferase Activity Colorimetric/Fluorometric Assay Kit
Cat.No. : | Kit-2209 |
Product Overview : | Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction:α-ketoglutarate + alanine; glutamate + pyruvate It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to;-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput activity assay of ALT with a detection limit of 10 mU per well. |
- Specification
- Gene Information
- Related Products
Applications : | Evaluation of pentose phosphate pathway. |
Storage : | -20°C |
Size : | 100 assays |
Publication : |
Suppression of Cisplatin-Induced Hepatic Injury in Rats Through Alarmin High-Mobility Group Box-1 Pathway by Ganoderma lucidum: Theoretical and Experimental Study (2020)
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Products Types
◆ Recombinant Protein | ||
ALT-116 | Recombinant ALT protein, His-tagged | +Inquiry |
◆ Assay kits | ||
Kit-0084 | ALT Fluorometric Assay Kit | +Inquiry |
Kit-0082 | Alanine Transaminase Assay Kit | +Inquiry |
Kit-0083 | ALT Assay Kit | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionALT expression is regulated by transcription factors, epigenetic modifications, and signaling pathways, such as Wnt/尾-catenin, MYC, or TGF-尾 signaling, providing insights into its regulation under different cellular conditions.
ALT interacts with various proteins, including transcription factors, chromatin remodelers, and co-regulators, influencing gene expression, DNA repair, or chromatin organization.
ALT plays essential roles in telomere maintenance, cellular senescence, telomere recombination, and alternative lengthening of telomeres (ALT) pathway activation.
ALT undergoes post-translational modifications, including phosphorylation, acetylation, and SUMOylation, which can modulate its activity, stability, and recruitment to telomeres or subcellular compartments.
ALT exhibits enzymatic activity as a DNA-dependent RNA polymerase, catalyzing the synthesis of RNA from a DNA template, but its specific substrates and metabolic pathways are still being elucidated.
Genetic alterations in ALT-related genes or mutations affecting telomere maintenance components are linked to telomere dysfunction disorders or predisposition to cancer development.
Therapeutic strategies targeting ALT or its associated pathways may hold potential for cancer therapy by exploiting ALT activation as a vulnerability or developing specific inhibitors to disrupt ALT activity and telomere maintenance in cancer cells.
Dysregulation of ALT has been implicated in various diseases, including cancer, where ALT activation is associated with telomere maintenance and genomic instability.
Structural studies have revealed key domains in ALT, such as the reverse transcriptase domain, telomerase essential N-terminal (TEN) domain, and RNA-binding motifs, providing insights into its function and catalytic mechanism.
ALT is primarily localized in the nucleus, but it can also be found in the cytoplasm and other cellular compartments, depending on the cell type and context.
Customer Reviews (3)
Write a reviewConvenient and efficient protein labeling options.
Reduced nonspecific binding for cleaner data interpretation.
Excellent resolution in protein separation techniques.
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