ATF2 (pT69/pT71) Transcription Factor Assay Kit
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Cat.No. : | Kit-0111 |
Product Overview : | ATF2 (pT69/pT71) Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the cAMP-response element (CRE) is immobilized onto the wells of a 96-well plate. ATF2 contained in a nuclear extract, binds specifically to the CRE. The activated ATF2 transcription factor complex is detected by addition of a specific primary antibody directed against the phospho-Thr(pT69/pT71) epitope on ATF2. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. |
Size : | 96 tests |
Applications : | Functional Studies more details. |
◆ Recombinant Protein
ATF2-838R | Recombinant Rat ATF2 Protein | +Inquiry |
ATF2-849H | Recombinant Human ATF2 Protein, His-tagged | +Inquiry |
ATF2-6466C | Recombinant Chicken ATF2 | +Inquiry |
ATF2-937H | Recombinant Human ATF2 protein, GST-tagged | +Inquiry |
ATF2-6867H | Recombinant Human ATF2, His & GST tagged | +Inquiry |
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◆ Lysates
ATF2-518HCL | Recombinant Human ATF2 cell lysate | +Inquiry |
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