B-Phycoerythrin Protein

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B-Phycoerythrin Protein

B-Phycoerythrin Related Products

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Cat.No. : B-Phycoerythrin-316
Product Overview : B-Phycoerythrin Protein, activated.
Description : B-Phycoerythrin, activated was treated with a 15-fold molar excess of 2-Iminothiolane.
Ratio SH-group/pigment: >= 3 (~5; Ellman’s reagent titration);
Fluorescence: Emmax 576 nm (exc.: 542 nm in 0.1 M phosphate pH 7.2);
Form : 1 vial contains 1 mg pigment in 0.2 mL buffer (0.1 M phosphate, 5 mM EDTA)
Applications : Suitable for fluorescence.
Activated B-Phycoerythrin is a reagent used to create B-Phycoerythrin type phycobiliproteins that may be analyzed via flow cytometry and immunofluorescence detection techniques.
Notes : Example for a labeling protocol:
1 mL purified at ~1.5 mg/mL in PBS (pH 7.5). Antibody must be fairly pure – i.e. purify on protein A or G column before conjugating. Add 20 µl SMCC, 4-(N-Maleimidomethyl)cyclohexanecarboxylic acid N-hydroxysuccinimide ester and incubate for 1 hour.
Conjugation with activated phycobiliprotein:
Immediately combine your activated phycobiliprotein with SMCC-Antibody.
NOTE: For activated PE start with a 1:1 molar ratio. For activated APC, start with a 2:1 molar ratio.
Incubate the mixture for 1-2 hours at room temperature. Block further reaction by adding 10:1 of NEM Solution(N-Ethylmaleimide) per mL of reaction (optional step). Incubate the mixture for 20 minutes at room temperature.
Use a desalting column to exchange the conjugate into the desired storage buffer. To optimize the yield and size distribution of the conjugate, alter the amount of activated-Phycobiliprotein to your protein.
Storage : Store activated B-Phycoerythrin in the dark at 0 until -20centigrade.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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