Bovine Intestinal Alkaline Phosphatase
Cat.No. : | BIAP-74B |
Product Overview : | The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity. Alkaline Phosphatase is Dimeric protein having a molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity. |
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Cat. No. : | BIAP-74B |
Source : | Calf Intestine. |
Physical Appearance : | Sterile Filtered clear colorless solution (11.1 mg/ml). |
Purity : | 95% pure by Gel Filtration. |
Formulation : | 50% glycerol, 5 mM MgCl2, 0.1 mM ZnCl2 and 5 mM TRIS, pH 7.0. |
Biological Activity : | >1500 U/mg (pH 9.6, 37°C and 0.025 M glycin, 10% glycerol as buffer). |
Stability : | AP although stable at 14°C for 1 weeks, should be stored at 4°C. Please Do-Not freeze. |
Synonyms : | EC 3.1.3.1; IAP; AP; Alk_phosphatase; glycerophosphatase; Kasahara isozyme; alkaline phosphatase, intestinal; alkaline phosphomonoesterase; intestinal alkaline phosphatase; alkaline phosphatase; alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum); phosphate-monoester phosphohydrolase (alkaline optimum). |
Synonyms : | EC 3.1.3.1; IAP; AP; Alk_phosphatase; glycerophosphatase; Kasahara isozyme; alkaline phosphatase, intestinal; alkaline phosphomonoesterase; intestinal alkaline phosphatase; alkaline phosphatase; alkaline phosphomonoesterase; phosphomonoesterase; glycerophosphatase; alkaline phosphohydrolase; alkaline phenyl phosphatase; orthophosphoric-monoester phosphohydrolase (alkaline optimum); phosphate-monoester phosphohydrolase (alkaline optimum). |
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