|Product Overview:||The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of environmental pollutants and cell mediated toxicity and studying programmed cell death (apoptosis). The assay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 - 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.|
|Compatible Sample Types:||Cell culture|
|Features & Benefits:||Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).
Sensitive and accurate. As low as 950 cells can be accurately quantified. Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.
Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
Robust and amenable to HTS. Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems.
|Assay time:||5 hrs|