Cholesterol Detection Kit (cell-based)

• Cat.No.: Kit-2317
• Product Overview: Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compar;ents is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol.

Specification

• Tag: Non
• Applications: Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: Fixative Solution; Assay Buffer; Staining Dye
• Detection method: Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm).
• Features & Benefits: Simple, easy to perform, histochemical method of identification of unesterified cholesterol.
  Includes U-18666A, a cholesterol transport inhibitor.