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| Cat.No. : | Kit-2317 |
| Product Overview : | Cholesterol is an essential structural component of animal cell membranes. It is required to maintain membrane structural integrity and fluidity. Cholesterol also serves as a precursor for the biosynthesis of several hormones, bile acid and vitamin D. Plasma membrane contains majority of the cellular cholesterol (80-90%) whereas little cholesterol resides in endoplasmic reticulum and in mitochondrial membrane. Transport of intracellular cholesterol within the cells to different compar;ents is through vesicular and non-vesicular pathways. Defects in these transport processes can alter cellular cholesterol metabolism resulting in pathological conditions. Filipin III is widely used as a probe for sterol localization in membranes. Interaction with cholesterol alters Filipin absorption and fluorescence spectra. This assay provides a simple, easy to perform, histochemical method of identification of unesterified cholesterol. |
| Applications : | Screen/study/characterize stimulators/inhibitors that affect cholesterol transport and localization in a variety of cells. |
| Storage : | -20°C |
| Shipping : | Gel Pack |
| Size : | 100 assays |
| Kit Components : | Fixative Solution; Assay Buffer; Staining Dye |
| Detection method : | Light and Fluorescence Microscopy (Ex/Em = 340-380/385-470 nm). |
| Features & Benefits : | Simple, easy to perform, histochemical method of identification of unesterified cholesterol. Includes U-18666A, a cholesterol transport inhibitor. |
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