"Chymotrypsin" Related Products

Chymotrypsin Activity Assay Kit (Fluorometric)

Cat.No.: Kit-1059
Description: Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine). Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin.
Applications: Measurement of chymotrypsin activity in tissues / cultured cells.
The assay can detect as low as 0.01 mU of Chymotrypsin in various samples.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: Chymotrypsin Assay Buffer; Chymotrypsin Substrate; Chymotrypsin Activator; Chymotrypsin Inhibitor; Coumarin Standard (1 mM); Chymotrypsin Positive Control
Target Species: Mammalian
Detection method: Fluorescence (Ex/Em= 380/460 nm)
Features & Benefits: Simpleprocedure; takes ~ 1-2 hours; Fast and convenient

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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