cPLA2 Assay Kit
Cat.No. : | Kit-0696 |
Product Overview : | Phospholipase A2 catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids, yielding a free fatty acid and a lysophospholipid as products. The release of arachidonic acid from membrane phospholipids by these enzymes is believed to be the key step in the biosynthesis of eicosanoids. There are primarily three different kinds of phospholipase A2. They are secretory (sPLA2), calcium-dependent cytosolic (cPLA2), and calcium-independent cytosolic (iPLA2) phospholipase A2. Of these three different types of enzymes, only the cPLA2 exhibits specificity towards arachidonic acid whereas all others can hydrolyze any fatty acid at the sn-2 position. Arachidonoyl Thio-PC is a substrate for cPLA2 by virtue of the presence of arachidonic acid at the sn-2 position of the glycerophospholipid. Hydrolysis of the arachidonoyl thioester bond at the sn-2 position by PLA2 releases a free thiol which can be detected by DTNB (5,5"-dithiobis (2-nitrobenzoic acid)). This assay can be used to determine the activity of cPLA2 in purified preparations, cell cultures, or tissue homogenates that are known to contain only cPLA2. Use of this assay with preparations containing more than one type of PLA2 will result in the measurement of total PLA2 activity rather than cPLA2 alone. Isozyme-specific cPLA2 activity can be measured by excluding sPLA2 or inhibiting iPLA2 activities in the assay. Each kit contains cPLA2 assay buffer, DTNB/EGTA, Arachidonoyl Thio-PC (substrate), bee venom PLA2 (control), bromoenol lactone solution, a 96 well plate, and complete instructions. |
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Storage : | Stability: 1 year;Storage: -20°C;This kit will perform as specified if stored at -20°C and used before the expiration date indicated on the outside of the box. |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, PLA2 inhibitors have been investigated for their potential in reducing cardiovascular risk, particularly in atherosclerosis.
PLA2 releases arachidonic acid, which serves as a precursor for pro-inflammatory eicosanoids, leading to inflammation.
PLA2 dysfunction is implicated in neurodegenerative diseases like Alzheimer's and Parkinson's, making it a target for research and potential therapies.
Genetic polymorphisms in PLA2 have been studied for their potential links to disease susceptibility, including inflammatory disorders.
Yes, some drugs inhibit PLA2 activity to manage inflammation, as seen in the treatment of rheumatoid arthritis.
Customer Reviews (3)
Write a reviewA manufacturer that can deliver the protein promptly and reliably can save researchers time and allow them to proceed with their experiments without unnecessary delays.
An PLA2 protein manufacturer that also offers related reagents such as antibodies or assay kits can streamline the research process.
This support could range from assisting with experimental design to offering advice on optimal protein handling and storage conditions.
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