| Species : |
Honey bee |
| Source : |
E.coli |
| Tag : |
Non |
| Protein Length : |
26-162 a.a. |
| Description : |
Bee venom phospholipase A2 (PLA) is the main allergen in the bee sting allergy. Bee venom phospholipase A2 (BV-PLA2) is a hydrolytic enzyme which specifically cleaves the sn-2 acyl bond of phospholipids at the lipid/water interface. BV-PLA2 is a 14–16-kDa glycoprotein, consisting of 134 amino acids and displaying a single carbohydrate side chain at the residue Asn13. It is also held to be responsible for some systemic anaphylactic reactions in bee venom sensitized individuals. BV-PLA2 presents 3 peptide and a glycopeptide T cell epitopes, which are recognized by both allergic and non-allergic bee venom sensitized subjects. PLA is able to elicit both IgE mediated allergy and normal immunity to bee sting which usually is associated with high affinity IgG4 anti-PLA antibodies. |
| Form : |
10mM Tris-HCl, pH 6.5, 0.1M Sodium Phosphate and 8M urea. |
| Purity : |
Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC. |
| Applications : |
Use as an antigen in ELISA and Western Blots. |
| Stability : |
Five years frozen. One month in solution at room temperature. |
| Storage : |
Protein is shipped at ambient temperature. Upon arrival, Store at -20°C. |
