D-2-Hydroxyglutarate Dehydrogenase Fluorometric Assay Kit

• Cat.No.: Kit-2108
• Product Overview: D-2-Hydroxyglutarate Dehydrogenase Assay kit provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples. In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces PicoProbe to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µl of D2HGDH activity.

Specification

• Size: 100 assays
• Applications: Measurement of D2HGDH activity in purified enzyme preparations, tissues and cells
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.D2HGDH Assay Buffer: Bring to room temperature before use. Store at 4°C or -20°C.PicoProbe: Before use, thaw at room temperature. Store at -20°C. Use within two months.D2HGDH Substrate: Store at -20°C, stable for 6 months.D2HGDH Developer: Reconstitute with 220 µl Assay Buffer. Pipette up and down to dissolve completely. Store at -20°C. Use within two months.D2HGDH Positive Control: Reconstitute with 100 µl Assay Buffer and mix thoroughly. Aliquot and store at -70°C. Avoid freeze/thaw. Use within two months. Keep on ice while in use.NADH Standard: Reconstitute with 200 μl dH2O to generate 1 mM (1 nmol/μl) NADH Standard solution. Aliquot and store at –20°C. Use within two months. Keep on ice while in use.