D-Gluconate (D-Gluconic Acid) Colorimetric Assay Kit
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|Product Overview :||D-Gluconate (Gluconic Acid) Assay kit is a sensitive, fast and easy-to-use kit. In this assay, Gluconate is utilized by Gluconokinase to form D-Gluconate-6-P and ADP, which subsequently undergoes a series of reactions to form an intermediate that reduces Gluconate probe to give a product with strong absorbance at 450 nm. This assay kit can detect D-Gluconate (DGluconic Acid) level less than 2 µM in a variety of samples.|
|Size :||100 assays|
|Applications :||Measurement of D-Gluconate (D-Gluconic Acid) in various samples such as tissues, plants, fruits and wine|
|Storage :||Store kit at -20°C, protect from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Gluconate Assay Buffer: Warm to room temperature before use.Gluconate Converter, Gluconate Enzyme Mix, and Gluconate Developer: Reconstitute each with 220 μl Assay Buffer. Pipette up and down to dissolve completely. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Keep on ice while in use. Use within two months.ATP and Gluconate Probe: Reconstitute with 220 μl dH2O. Pipette up and down to completely dissolve. Store at -20°C. Use within two months.Gluconate Standard: Reconstitute with 100 μl dH2O to generate 100 mM (100 nmol/μl) Standard solution. Store at -20°C. Use within two months. Keep on ice while in use.|