Recombinant Human Thiamine Triphosphatase, His-tagged THTPA-786H

Recombinant Human Thiamine Triphosphatase, His-tagged

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Recombinant Human Thiamine Triphosphatase, His-tagged

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Cat. No. : THTPA-786H
Product Overview : Recombinant human THTPA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 27.7 kDa (250aa) confirmed by MALDI-TOF(Real molecular weight on SDS-PAGE will be shift up).
Description : THTPA, also known as THTP or THTPASE, is a member of the THTPase family. This protein is localized to the cytoplasm and expressed at low levels in a variety of tissues, including testis, uterus, prostate, bladder, lung and kidney. THTPA is a hydrolase that catalyzes the H2O-dependent hydrolysis of thiamine triphosphate (THTP) to thiamine diphosphate (THDP), the major form of thiamine within the cell. THTPA exists as a monomer and functions at an optimal pH of 8.5.
Source : E.coli.
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MAQGLIEVER KFLPGPGTEE RLQELGGTLE YRVTFRDTYY DTPELSLMQA DHWLRRREDS GWELKCPGAA GVLGPHTEYK ELTAEPTIVA QLCKVLRADG LGAGDVAAVL GPLGLQEVAS FVTKRSAWKL VLLGADEEEP QLRVDLDTAD FGYAVGEVEA LVHEEAEVPT ALEKIHRLSS MLGVPAQETA PAKLIVYLQR FRPQDYQRLL EVNSSRERPQ ETEDPDHCLG
Purity : > 90% by SDS – PAGE.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT.
Concentration : 1 mg/ml (determined by Bradford assay).
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : THTPA thiamine triphosphatase [ Homo sapiens ]
Synonyms : THTPA; thiamine triphosphatase; THTP; MGC2652; THTPASE; thiamine-triphosphatase; EC 3.6.1.28
Gene ID : 79178
mRNA Refseq : NM_001126339
Protein Refseq : NP_001119811
MIM : 611612
UniProt ID : Q9BU02
Chromosome Location : 14q11.2
Pathway : Metabolic pathways; Thiamine metabolism
Function : adenylate cyclase activity; hydrolase activity; thiamin-triphosphatase activity

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