Recombinant Human Neutrophil Cytosolic Factor 1, His-tagged

Cat. No.: HIF1A-2773H
Product Overview: Recombinant human HIF1A(576-785) protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 25.1 kDa (231aa).
Description: HIF1A, identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. This protein is a heterodimer composed of HIF-1beta subunit and one of three subunits(HIF-1A, HIF-2A or HIF-3A). The activation of HIF1A is closely associated with a variety of tumors and oncogenic pathways. HIF1A consists of DNA binding domain(DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains.
Source: E.coli.
Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol.
Purity: > 85% by SDS - PAGE.
Concentration: 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSFDQLSPLE SSSASPESAS PQSTVTVFQQ TQI QEPTANA TTTTATTDEL KTVTKDRMED IKILIASPSP THIHKETTSA TSSPYRDTQS RT A SPNRAGK GVIEQTEKSH PRSPNVLSVA LSQRTTVPEE ELNPKILALQ NA QRK RKMEH DGSLFQAVGI GTLLQQPDDH AATTSLSWKR VKGCKSSEQN GME QKTIILI PSDLACRLLG Q.
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) [ Homo sapiens ]
Synonyms: HIF1A; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; Hypoxia-inducible factor 1 alpha; HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; member of PAS superfamily 1; hypoxia-inducible factor 1, alpha subunit
Gene ID: 3091
mRNA Refseq: NM_001530
Protein Refseq: NP_001521
MIM: 603348
UniProt ID: Q16665
Chromosome Location: 14q21-q24
Pathway: Pathways in cancer; Renal cell carcinoma; mTOR signaling pathway
Function: Hp90 protein binding; RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein heterodimerization activity; sequence-specific DNA binding; signal transducer activity; transcription factor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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