Active Recombinant E. coli AmpC protein, His-tagged

• Cat.No.: ampC-967E
• Product Overview: Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli.

Specification

• Description: AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Bio-activity: Specific activity is >700unit/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37 centigrade.
• Molecular Mass: 41.8 kDa
• Protein length: 20-377aa
• AA Sequence: APQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at 2 centigrade to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 centigrade to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml

Gene Information

• Gene Name: ampC beta-lactamase [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: ampC
• Synonyms: ampC; ampA; Cephalosporinase
• Gene ID: 948669
• Protein Refseq: NP_418574
• UniProt ID: P00811