Recombinant Human SF3B14, His-tagged

• Cat.No.: SF3B14-3411H
• Product Overview: Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Cat. No.: SF3B14-3411H
• Description: SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex.
• Source: E.coli.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, and 1mM EDTA, 30% glycerol.
• Molecular Weight: 16.7 kDa (145aa) confirmed by MALDI-TOF.
• Purity: > 95% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEE MYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Spliceosome; Gene Expression; mRNA Processing

Gene Information

• Gene Name: SF3B14 splicing factor 3B, 14 kDa subunit [ Homo sapiens ]
• Synonyms: splicing factor 3B, 14 kDa subunit; P14; Ht006; SAP14; CGI-110; HSPC175; SF3B14a;pre-mRNA branch site protein p14; SF3b 14 kDa subunit; spliceosome-associated protein, 14 kDa subunit
• Gene ID: 51639
• mRNA Refseq: NM_016047
• Protein Refseq: NP_057131
• MIM: 607835
• UniProt ID: Q9Y3B4
• Chromosome Location: 2pter-p25.1
• Function: RNA binding; nucleotide binding; protein binding