Recombinant Human MB protein, His-tagged

• Cat.No.: MB-3429H
• Product Overview: Recombinant Human MB(1-154aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Myoglobin, mitochondrial precursor, also known as MB, is a cytosolic oxygen-binding protein responsible for the storage and diffusion of oxygen within myocytes. Expression of myoglobin is highest in skeletal and cardiac muscle. MB may serve a variety of functions in muscular oxygen supply, such as oxygen storage, facilitated diffusion, and myoglobin-mediated oxidative phosphorylation.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
• Molecular Mass: 19.3 kDa (174aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSHTALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH PGDFGADAQG AMNKALELFR KDMASNYKEL GFQG MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE DLKKHGATVL
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MB myoglobin [ Homo sapiens ]
• Official Symbol: MB
• Synonyms: MB; myoglobin; PVALB; MGC13548
• Gene ID: 4151
• mRNA Refseq: NM_203378
• Protein Refseq: NP_976312
• MIM: 160000
• UniProt ID: P02144
• Chromosome Location: 22q13.1
• Function: heme binding; metal ion binding; oxygen binding; oxygen transporter activity