||Recombinant human ARF1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 22.8 kDa (201aa).
||ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT.
||> 90% by SDS - PAGE.
||1.0 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTIL YK LKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGGQDKIRPLWRH YFQNTQGLIF VV DSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKL GLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Vibrio cholerae infection; HIV Infection; Membrane Trafficking