||Recombinant human RAB21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Ras-related protein Rab-21, also known as RAB21, is a member of the Ras superfamily of small GTP-binding proteins. Rab21 is integrin-associated protein and positive regulators of integrin traffic. This protein associate with the cytoplasmic domains of alpha-integrin chains and their expression influences the endo/exocytic traffic of integrins. This function of Rab21 is dependent on its GTP/GDP cycle and proper membrane targeting.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 200mM NaCl, 1mM PMSF
||27.1kDa (242aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMYSF KVVLLGEGCV GKTSLVLRYC ENKFNDKHIT TLQASFLTKK LNIGGKRVNL AIWDTAGQER FHALGPIYYR DSNGAILVYD ITDEDSFQKV KNWVKELRKM LGNEICLCIV GNKIDLEKER HVSIQEAESY AESVGAKHYH TSAKQNKGIE ELFLDLCKRM IETAQVDERA KGNGSSQPGT ARRGVQIIDD EPQAQTSGGG CC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.