||Recombinant human CAPS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||Calcyphosine, also known as CAPS, is a calcium-binding protein containing four EF-hand domains. CAPS is initially identified as thyroid protein p24 that is found in several epithelium and in some cells of the central nervous system. It may play a role in the regulation of ion transport. In thyroid follicular cells, CAPS is synthesized and phosphorylated in response to stimulation by thyrotropin and cAMP agonists.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl
||23.1kDa (209aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MDAVDATMEK LRAQCLSRGA SGIQGLARFF RQLDRDGSRS LDADEFRQGL AKLGLVLDQA EAEGVCRKWD RNGSGTLDLE EFLRALRPPM SQAREAVIAA AFAKLDRSGD GVVTVDDLRG VYSGRAHPKV RSGEWTEDEV LRRFLDNFDS SEKDGQVTLA EFQDYYSGVS ASMNTDEEFV AMMTSAWQL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.