||Recombinant human UFM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 and E2-like conjugating enzyme UFC1 in a manner analogous to ubiquitylation. It localizes primarily to the nucleus, but is also present in diffuse amounts in the cytoplasm. This protein is expressed in a variety of tissues, including kidney, brain, heart, liver and lung.
||Liquid. In 20mM Tris buffer(pH 8.0) containing 10% glycerol.
||11.1 kDa (103aa), confirmed by MALDI-TOF
||> 95 % by SDS - PAGE
||1 mg/ml (determined by BCA assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSKVSFKITL TSDPRLPYKV LSVPESTPFT AVLKFAAEEF KVPAATSAII TNDGIGINPA QTAGNVFLKH GSELRIIPRD RVG
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.