"NCBP2" Related Products

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Recombinant Human NCBP2, His-tagged

Cat. No.: NCBP2-2847H
Product Overview: Recombinant human NCBP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =20.1 kDa (176aa), confirmed by MALDI-TOF.
Description: NCBP2, also known as CBC2, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5" cap of nascent pre-mRNA in the nucleoplasm. This protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3"-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.
Source: E.coli.
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ
Purity: > 95% by SDS – PAGE.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
Concentration: 0.5 mg/ml (determined by Bradford assay).
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: NCBP2 nuclear cap binding protein subunit 2, 20kDa [ Homo sapiens ]
Synonyms: NCBP2; nuclear cap binding protein subunit 2, 20kDa; CBC2; NIP1; CBP20; PIG55; nuclear cap-binding protein subunit 2; OTTHUMP00000208156; OTTHUMP00000208157; OTTHUMP00000208158; NCBP 20 kDa subunit; NCBP interacting protein 1; NCBP-interacting protein 1; 20 kDa nuclear cap-binding protein; cell proliferation-inducing gene 55 protein
Gene ID: 22916
mRNA Refseq: NM_001042540
Protein Refseq: NP_001036005
MIM: 605133
UniProt ID: P52298
Chromosome Location: 3q29
Pathway: Spliceosome
Function: RNA 7-methylguanosine cap binding; NOT RNA binding; RNA cap binding; nucleotide binding; protein binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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