||Recombinant human SCAND1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 21.2 kDa (199aa) confirmed by MALDI-TOF (molecular size on SDS-PAGE will appear higher).
||SCAN domain-containing protein 1, also known SCAND1, belongs to a family of genes that encode an isolated SCAN domain. SCAND1 contains one SCAN box domain and, unlike the majority of other SCAN domain containing proteins, it does not contain a zinc finger motif. SCAND1 is a nuclear protein that may play a role in the mechanism of transcription regulation. It is widely expressed with highest levels of expression found in kidney, thyroid, liver, prostate and testis.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MAATEPILAA TGSPAAVPPE KLEGAGSSSA PERNCVGSSL PEASPPAPEP SSPNAAVPEA IPTPRAAASA ALELPLGPAP VSVAPQAEAE ARSTPGPAGS RLGPETFRQR FRQFRYQDAA GPREAFRQLR ELSRQWLRPD IRTKEQIVEM LVQEQLLAIL PEAARARRIR RRTDVRITG
||> 90% by SDS – PAGE.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.